osteoblast growth medium bulletkit (ogm)  (Lonza)

Journal: Materials

Article Title: Influence of Low Temperature Plasma Oxidizing on the Bioactivity of NiTi Shape Memory Alloy for Medical Applications

doi: 10.3390/ma16186086

Figure Lengend Snippet: Proliferation of osteoblasts cultured on NiTi in the initial state and with a TiO 2 surface layer represented as the number of cells per mm 2 and measured after 24 h, 48 h and 6 days of growth; * p ≤ 0.05 NiTi versus NiTi + TiO 2 .

Article Snippet: The cells were grown in an osteoblast basal medium enriched with fetal bovine serum (50 mL/500 mL medium), ascorbic acid (0.5 mL/500 mL medium), GA-1000 (0.5 mL/500 mL medium) antibiotics (OGM Osteoblast Growtth Medium BulletKit, Lonza), in a humid atmosphere of 95% air and 5% CO 2 at 37 °C.

Techniques: Cell Culture

Journal: Materials

Article Title: Influence of Low Temperature Plasma Oxidizing on the Bioactivity of NiTi Shape Memory Alloy for Medical Applications

doi: 10.3390/ma16186086

Figure Lengend Snippet: Morphology of osteoblasts cultured on the surfaces of NiTi alloy in the initial state ( a , a’ , c , c’ , e , e’ ) and with a TiO 2 surface layer ( b , b’ , d , d’ , f , f’ ) for 24 h ( a , a’ , b , b’ ), 48 h ( c , c’ , d , d’ ) and 6 days ( e , e’ , f , f’ ). SEM images magnified ×60 ( a – f ), ×500 ( a’ – f’ ).

Article Snippet: The cells were grown in an osteoblast basal medium enriched with fetal bovine serum (50 mL/500 mL medium), ascorbic acid (0.5 mL/500 mL medium), GA-1000 (0.5 mL/500 mL medium) antibiotics (OGM Osteoblast Growtth Medium BulletKit, Lonza), in a humid atmosphere of 95% air and 5% CO 2 at 37 °C.

Techniques: Cell Culture

Journal: Materials

Article Title: Hydroxyapatite from Natural Sources for Medical Applications

doi: 10.3390/ma15155091

Figure Lengend Snippet: Cell viability of osteoblasts for the 12 HAp samples. The stock solution was diluted in binary dilutions (1/2, 1/4, and 1/8).

Article Snippet: The 12 tested samples were placed in a 12-well cell culture plate, with growth medium completely specific to the osteoblast cell line (OGM TM Osteoblast Growth medium BulletKit TM , Lonza, Germany), and incubated for 24 h at 37 °C, in a shaking incubator, 5% CO 2 .

Techniques:

Journal: Materials

Article Title: Hydroxyapatite from Natural Sources for Medical Applications

doi: 10.3390/ma15155091

Figure Lengend Snippet: Cell proliferation of osteoblasts on HAp samples.

Article Snippet: The 12 tested samples were placed in a 12-well cell culture plate, with growth medium completely specific to the osteoblast cell line (OGM TM Osteoblast Growth medium BulletKit TM , Lonza, Germany), and incubated for 24 h at 37 °C, in a shaking incubator, 5% CO 2 .

Techniques: